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BBiologyS2 to S6Plant transport

Transpiration lab: a bubble potometer

A big tree can lose hundreds of litres of water on a hot day. Use a potometer to find what makes leaves lose water faster.

← bubble movesvolume = π r² d
LevelS2 to S6 (and IGCSE, A Level)
TimeAbout 40 min
You useπ r² d, rates, percentages
PriceFree: no account

🎯 Aim

To investigate how light, air movement, humidity and temperature affect the rate of transpiration using a bubble potometer.

You will learn to

  • Calculate the volume of water taken up from the bubble distance.
  • Calculate rates and percentage changes.
  • Explain each factor using stomata and diffusion gradients.
  • State the limitations of a potometer.

🧭 What to do

  1. Start with Bright light, Still air, Dry room, 25 °C.
  2. Tap Time 5 minutes and read the distance moved by the bubble.
  3. Change one condition only, then time 5 minutes again.
  4. Compare the rates on the bar graph.
  5. Answer the questions with the class data.
  6. Write your conclusion and download your lab report.

🔬 Lab bench

📋 The data

Distance moved by the bubble in 5 minutes (capillary radius 0.5 mm) in five conditions.

🧠 Investigate: answer from the data

Type your answer and tap Check. Use 💡 Hint first; open the method only when you are stuck.

📄 My lab report

Your report collects the aim, your data table, the graphs, your answers and your conclusion. It is made on your phone or computer: nothing is sent to anyone.

Check your conclusion

Transpiration is fastest in bright light, moving air, dry air and higher temperature: light opens the stomata, wind and dry air keep the water vapour gradient steep, and heat speeds up evaporation and diffusion. A plastic bag (humid air) and darkness slow it down. The potometer measures water uptake, which is very close to water lost.

➕ Get more from this experiment

📝 Free

Print the worksheet (button above), download the data, and save your report. Teachers can project this page in class.

📦 Premium Lab Pack

1 000 RWF

  • Practical worksheet for a home-made potometer
  • Recording tables and rate calculations
  • Teacher guide and answer key
  • Exam-style plant transport questions with marking scheme

👩‍🏫 For teachers

Use it as a practical when apparatus is missing, as homework, or as a project. Free lesson plans help you plan, teach and assess.

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