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Gene technology lab: PCR, gels and DNA profiling

Farmers improve their herds and doctors make insulin with gene technology. Copy DNA, cut it, and read a gel like a real laboratory.

🧫2ⁿ copies after n cyclesPCR, gels and DNA profiling
LevelS6 (and A Level)
TimeAbout 35 min
You usePowers of 2, logarithms
PriceFree: no account

🎯 Aim

To investigate PCR, restriction enzymes and gel electrophoresis and their uses.

You will learn to

  • Calculate DNA copies after PCR cycles.
  • Find fragment sizes from restriction sites.
  • Interpret gels using a ladder.
  • Discuss uses of gene technology.

🧭 What to do

  1. Move the Number of PCR cycles and read the copies.
  2. Choose Cut a plasmid and each Restriction enzyme.
  3. Compare the bands with the ladder.
  4. Choose Which bull is the father? and compare the bands.
  5. Answer the questions with the class data.
  6. Write your conclusion and download your lab report.

🔬 Lab bench

📋 The data

DNA profiles of a cow, her calf and three bulls.

🧠 Investigate: answer from the data

Type your answer and tap Check. Use 💡 Hint first; open the method only when you are stuck.

📄 My lab report

Your report collects the aim, your data table, the graphs, your answers and your conclusion. It is made on your phone or computer: nothing is sent to anyone.

Check your conclusion

PCR doubles the DNA every cycle (2ⁿ copies) using primers and heat-stable Taq polymerase. Restriction enzymes cut at specific sequences, often leaving sticky ends. In electrophoresis negatively charged DNA moves towards the positive electrode, with small fragments moving furthest. Every band of an offspring must come from one of its parents.

➕ Get more from this experiment

📝 Free

Print the worksheet (button above), download the data, and save your report. Teachers can project this page in class.

📦 Premium Lab Pack

1 000 RWF

  • Worksheet: paper gel electrophoresis and an ethics debate on GMOs
  • Gene technology problems
  • Teacher guide and answer key
  • Exam-style questions with marking scheme

👩‍🏫 For teachers

Use it as a practical when apparatus is missing, as homework, or as a project. Free lesson plans help you plan, teach and assess.

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DONAT STEM Interactive Labs · original work by BIZIYAREMYE Donat · data are realistic models for learning · free forever